Regulatory

Part:BBa_J100444

Designed by: Mira V Hartshorn   Group: Campbell M Lab   (2018-09-05)


3-6 base change

Our group mutated two bases of the original B21 RBS sequence. The third base of the RBS sequence was changed from an A to G in order to form three hydrogen bonds (Watson-Crick bond) with the corresponding C of the 16S rRNA. It is predicted that this change in base will create a stronger and more stable bond. The sixth base of the RBS sequence was changed from a G to a C. This will ideally decrease the number of nucleotides in the ring. Both of these changes are predicted to stabilize the structure while maintaining optimal efficiency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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